Shahdeo, Deepshikha and Kesarwani, Veerbhan and Suhag, Deepa and Ahmed, Jahangeer and Alshehri, Saad M. and Gandhi, Sonu (2021) Self-assembled chitosan polymer intercalating peptide functionalized gold nanoparticles as nanoprobe for efficient imaging of urokinase plasminogen activator receptor in cancer diagnostics. Carbohydrate Polymers, 266. p. 118138. ISSN 01448617

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Abstract

Targeting cell surface receptors for specific drug delivery in cancer has garnered lot of attention. Urokinase plasminogen activator receptor (uPAR), a surface biomarker, is overexpressed on many tumours including breast, colorectal, prostate, and ovarian cancers. Binding of growth factor domain (GFD) of urokinase plasminogen activator (uPA) with uPAR lead to its close conformation, and allow somatomedin B domain (SMB) of vitronectin binding by allosteric modulation. In-silico docking of uPAR with GFD and SMB peptides was performed to identify potential binding affinity. Herein, we report fluorescently labeled peptide functionalized AuNPs with a mixed self-assembled monolayer of intercalating chitosan polymer for efficient targeting and imaging of uPAR-positive cells. The biophysical characterization of nanoconjugates and uPAR-specific targeting was assessed by FACS, cell adhesion, and fluorescence imaging. AuNPs/chitosan/GFD+SMB peptides showed higher uptake as compared to AuNPs/chitosan/GFD, and AuNPs/chitosan/SMB that can be utilized as a tool for molecular targeting and imaging in metastasis.

Item Type:	Article
Subjects:	Animal Genetics and Genomics Infectious Diseases Inflammation Biology Reproductive Biology Genetic Engineering Bioinformatics
Depositing User:	Mr Harjit Singh
Date Deposited:	02 Feb 2023 05:35
Last Modified:	02 Feb 2023 05:35
URI:	http://niab.sciencecentral.in/id/eprint/104

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