Annamanedi, Madhavi and Sheela, P. and Sundareshan, Srinivasaiah and Isloor, Shrikrishna and Gupta, Priya and Jasmeen, Pagala and Gargi, Madhuranjana and Mallick, Sathi and Hegde, Nagendra R. (2021) Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India. Scientific Reports, 11 (1). ISSN 2045-2322

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Abstract

Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly from the Southern State of Karnataka, but also from a few other states. The results revealed (a) 8 to 13 fragments in pulsed-field gel electrophoresis (PFGE), forming 31 distinct patterns, and (b) 34 spa types, of which three (t17680, t18314, and t18320) were newly identified. Multi-locus sequencing typing (MLST) identified 39 sequence types (STs), with ST2454 (34.4%) and ST2459 (24%) being the most commonly represented, which clustered to clonal complexes (CC) CC9 and CC97, respectively; 12 STs were newly identified. Thirty-four (20.5%) of the 166 isolates displayed oxacillin resistance. On the other hand, whereas none were mecC+, 44 (26.5%) isolates were mecA+, with a predominance of SCCmecIVb (26/32 isolates, others being untypeable); 24 isolates (14.46%) were oxacillin-susceptible methicillin-resistant S. aureus (OS-MRSA; mecA+ but OS). Integrated analysis revealed that CC9-ST2454- and CC97-ST2459-SCCmecIVb were the predominant MRSA, although the distribution of CC9 and CC97 was similar between methicillin-resistant and -susceptible isolates. By PCR, 56.25%, 28.75% and 47.5% of the 166 isolates were positive for hlg, tsst and pvl genes, respectively. Our results, for the first time describe the application of a combination of various molecular methods to bovine mastitis-associated S. aureus isolates from India, corroborate the worldwide distribution of CC97 and CC9, and suggest pathogenic potential of the isolates.

Item Type:	Article
Subjects:	Animal Genetics and Genomics Infectious Diseases Inflammation Biology Reproductive Biology Genetic Engineering Bioinformatics
Depositing User:	Mr Harjit Singh
Date Deposited:	02 Feb 2023 06:23
Last Modified:	02 Feb 2023 06:25
URI:	http://niab.sciencecentral.in/id/eprint/105

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