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Mitra, Pallabi and Deshmukh, Abhijit S. and Gurupwar, Rajkumar and Kashyap, Poonam (2019) Characterization of Toxoplasma gondii Spt5 like transcription elongation factor. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1862 (2). pp. 184-197. ISSN 18749399

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Elongation has emerged as a highly regulated step in the multistage process of transcription. Control of gene expression mediated through transcription elongation remains an unexplored area of study in Toxoplasma gondii where the demands of complex lifecycle necessitates a regulated transcription program. Here, we elucidate the central role of Spt5 homolog in T.. gondii mRNA transcription. We demonstrate that TgSpt5 functions in conjunction with a small zinc finger protein TgSpt4. TgSpt5 interacts with TgRpb1, the largest subunit of RNA polymerase II and associates with actively transcribed genes. Enrichment of TgSpt5 towards the 3’ end of genes coinciding with P-Ser2 form of RNAPII, a marker of active elongation further underscores its pivotal role in transcription. TgSpt5 undergoes phosphorylation mediated through Toxoplasma Cdk9 homolog, TgCrk9, which appears crucial for its function. Inhibition of TgCrk9, which also regulates RNAPII by differential phosphorylation of its C terminal domain, results in loss of TgSpt5 enrichment at 3’ sites of the genes and an overall repressive effect on parasite progression. TgSpt5 along with TgSpt4 could successfully complement the loss of function mutations in yeast counterparts emphasizing its functional significance. Together, the results highlight the possible role of TgSpt5 in transcript elongation regulated through phosphorylation by TgCrk9.

Item Type: Article
Subjects: Animal Genetics and Genomics
Infectious Diseases
Inflammation Biology
Reproductive Biology
Genetic Engineering
Depositing User: Mr Harjit Singh
Date Deposited: 26 Jun 2019 09:11
Last Modified: 15 Jul 2019 08:45
URI: http://niab.sciencecentral.in/id/eprint/72

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