Rajkhowa, Tridib Kumar and Thanga, Lalnun and Hauhnar, Lalthapui and Zodinpui, Doris and Subbiah, Madhuri (2022) Molecular detection and characterization of highly pathogenic porcine reproductive and respiratory syndrome virus from a natural outbreak in wild pigs, Mizoram, India. Transboundary and Emerging Diseases, 69 (4). ISSN 1865-1674

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Abstract

Thisstudyreportsforthefirsttimeanaturaloutbreakofhighlypathogenicporcinereproductive and respiratory syndrome (HP-PRRS) caused by HP-PRRS virus (HP-PRRSV)inwildpigscharacterizedbysuddenonsetofdepression,anorexia,respira-torydistress,andhighfever.Thediseasehascausedseverehaemorrhagicpneumonia,haemorrhagiclymphadenitis,enlargedspleenwithareasofinfarction,andpetechialhaemorrhages on the myocardium and on the surface of kidneys. HP-PRRSV wasdetectedinrepresentativetissuesamplesbyreversetranscription-PCR,andthefieldstrainwasisolatedintheMA104cellline.Thephylogeneticanalysesbasedonthewholegenomesequencesandnucleotidesequencesofopenreadingframe5(ORF5)geneshowedclosegroupingwiththesubtypeIVoflineage8/8.7ofPRRSVII,whichrepresentstheHP-PRRSVstrainsthatpredominateinthepigpopulationofChinasince2010.TheaminoacidsequenceanalysisoftheORF5generevealedthereplacementofleucine(L)atposition39toisoleucine(I)intheprimaryneutralizingepitope.AmongthefourpotentialNglycosylationsites,theN34wasmutatedandfoundtoberestrictedtoonlythreeNglycosylationsites.ThepresentfindingshaveindicatedthatHP-PRRSVcancausefataloutbreaksandmayemergeasamajorthreattothewildpigpopulation.

Item Type:	Article
Subjects:	Animal Genetics and Genomics
Depositing User:	Mr Harjit Singh
Date Deposited:	02 Feb 2023 06:55
Last Modified:	02 Feb 2023 06:55
URI:	http://niab.sciencecentral.in/id/eprint/107

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