Kannaki, T. R. and Priyanka, E. and Nishitha, Y. and Krishna, S. Vamshi and Haunshi, Santosh and Subbiah, Madhuri (2022) Molecular detection and phylogenetic analysis of Marek's disease virus virulence‐associated genes from vaccinated flocks in southern India reveals circulation of virulent MDV genotype. Transboundary and Emerging Diseases, 69 (4). ISSN 1865-1674

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Abstract

Marek’s disease (MD) is a re-emerging viral disease of chickens and a serious eco-nomicthreattothepoultryindustryworldwide.Continuoussurveillancewithmolec-ularinvestigationisessentialtomonitortheemergenceofvirulentMarek’sdiseasevirus(MDV)strainsandtodeviseanyappropriatevaccinationstrategyandimplementbio-securityprogrammes.Inthepresentstudy,weinvestigatedthecasesofMDout-breaksinvaccinatedpoultryflocks.TheMDoutbreakwasconfirmedthroughnecropsy(mainlyvisceraltumours),histopathologyandviralgenespecificPCR.Thepathotypesof the field MDV strains were assessed by molecular analysis of three virulence-associatedgenes,meq,pp38andvIL-8.TheMeqsequenceofthefieldstrainsanalyzedinthisstudylackedthe59aauniquetomildstrains,indicatingthattheyarepotentiallyvirulentstrains.Mutationatposition71andthepresenceoffiveprolinerichrepeatsinthetransactivationdomain,bothassociatedwithvirulencewereobservedinthesestrains;however,thesignaturesequencesspecifictoveryvirulentplusstrainswereabsent.PhylogeneticanalysisofmeqoncogenesequencesrevealedclusteringofthefieldstrainswithNorthIndianstrainsandwithaveryvirulentplusATE2539strainfromHungary.Analysesofpp38proteinatpositions107and109andvIL-8proteinatpositions4and31showedsignaturesofvirulence.Sequenceandphylogeneticanal-ysisofoncogeneandvirulence-associatedgenesoffieldMDVsfromvaccinatedflockindicatedthatthesestrainspossessedmolecularfeaturesofvirulentstrains.

Item Type:	Article
Subjects:	Animal Genetics and Genomics
Depositing User:	Mr Harjit Singh
Date Deposited:	02 Feb 2023 07:08
Last Modified:	02 Feb 2023 07:08
URI:	http://niab.sciencecentral.in/id/eprint/108

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